The effect of conservation in various cryoprotectants on the properties and morphological structure of the umbilical cord arteries

Purpose of the study . The aim this work was to effect conservation in various cryoprotectants on biomechanical and morphological properties umbilical cord (UCA) arteries. Materials methods material for 12 arteries, which were divided into 4 groups: (1) native UCA – control without freezing; (2) APC frozen 5 % dextran with addition dimethyl sulfoxide (DMSO) 10 7.5 human serum albumin (HSA); (3) glycerin (4) propanediol. For all groups, studies carried out. statistical data processed using GraphPad Prism 9.2.0 program. Analysis variance used find significant differences. Results Freezing dextran, DMSO did not lead changes morphology vascular tissue compared control. conserved retains a moderate level type I collagen, regardless method conservation. presence statistically differences between UCAs shown Young's modulus (p < 0.05; 6.9 [6.8; 7.0] artery; 2.3 [2.0; 2.6] artery preserved use albumin; [1.4; 3.2] 0.8 [0.6; 1.0] propanediol, respectively). Conclusion 1) storage at HSA does change membranes cell nuclei; 2) preservation collagen according results IHC is qualitatively reduced from conditional indicator +2 +1 agroindustrial complexes ones; 3) Conservation leads decrease elasticity walls complex vessels 0.05).